Rani Saira Saleem

Holds Masters degree in Biochemistry / Molecular Biology (2008) & M.Phil in Human Molecular Genetics, from Quaid-I-Azam University Islamabad, Pakistan (2010). Bachelor of Science in Chemistry, Botany & Zoology from University of Punjab Lahore, Pakistan (2004). Has research experience in the area of Human Molecular Genetics with emphasis on following topic: Mapping of genes responsible for Skeletal disorders; during which she was engaged in Genotyping/Mapping, identification and Sequence analysis of loci and genes involved in inheritance of postaxial polydactyly (Limb malformation). Has experience in molecular biology techniques: Clinical evaluation of patients and blood sampling. Genomic DNA extraction by phenol and kit method, DNA purification. RNA extraction and cDNA synthesis. PCR, Real Time PCR, RFLP, Electrophoresis. Enzyme assays, Chromatographic techniques. Basic laboratory equipment such as centrifuge, homogenizer, incubators, autoclaving, laminar flow hood, rotary evaporators, pH meter, spectrophotometer and Nanodrop. Also used bioinformatic tools & software for statistical analysis, evaluation and interpretation of the results. She has vast handling experience on all instruments of Bio-Rad Laboratories USA including RT-PCR, GelDOC XR, Automated electrophoresis systems, HPLC and Protein analyzers. She also holds teaching experience in the fields of biology and Chemistry.

Holds Masters degree in Biochemistry / Molecular Biology (2008) & M.Phil in Human Molecular Genetics, from Quaid-I-Azam University Islamabad, Pakistan (2010). Bachelor of Science in Chemistry, Botany & Zoology from University of Punjab Lahore, Pakistan (2004). Has research experience in the area of Human Molecular Genetics with emphasis on following topic: Mapping of genes responsible for Skeletal disorders; during which she was engaged in Genotyping/Mapping, identification and Sequence analysis of loci and genes involved in inheritance of postaxial polydactyly (Limb malformation). Has experience in molecular biology techniques: Clinical evaluation of patients and blood sampling. Genomic DNA extraction by phenol and kit method, DNA purification. RNA extraction and cDNA synthesis. PCR, Real Time PCR, RFLP,2D Gel Electrophoresis. Enzyme assays, Chromatographic techniques. Iso Electric Focusing (IEF), Liquid Chromatography, Basic laboratory equipment such as centrifuge, homogenizer, incubators, autoclaving, laminar flow hood, rotary evaporators, pH meter, spectrophotometer and Nanodrop. Also used bioinformatic tools & software for statistical analysis, evaluation and interpretation of the results. She has vast handling experience on all instruments of Bio-Rad Laboratories USA including RT-PCR, GelDOC XR, Automated electrophoresis systems, HPLC and Protein analyzers. She has COMPUTER SKILLS IN GENOME BIOLOGY: Genomics Data Mining by searching different online databases like NCBI, UCSC & Ensemble Data base etc; PRIMER-DESIGNING Softwares like (Oligo Explorer, Rutger s map & Primer3 etc); Linkage analysis Softwares like (easy LINKAGE, PEDCHECK & SUPERLINK etc) and Data Analysis Softwares like (Bio-Edit & other online Sequence Alignment tools etc) COMPUTER SKILLS IN PROTEOMICS: Proteomics Data Mining by searching different online databases like NCBI, Expasy, GelBank, Cornea 2D-PAGE, World 2DPAGE etc. Applying different Bio-softwares like Gel analysis softwares e.g. Image Master. She also holds teaching experience in the fields of biology and Chemistry.